Muaj cov kab mob nyob rau saum npoo ntawm ntau yam khoom yas hauv tsev kho mob, suav nrog cov nplooj ntawv thiab cov xov tooj ntawm tes. Peb lub tsev kho mob txhawb cov neeg ua haujlwm kho mob siv cov tshuaj tua kab mob hauv khw muag khoom los ntxuav cov khoom yas. Muaj pov thawj tias siv cov tshuaj tua kab mob raws li cov lus qhia tuaj yeem txo cov kab mob zoo. Txawm li cas los xij, nws tau pom tias txoj kev cov neeg ua haujlwm kho mob siv cov tshuaj tua kab mob sai heev, thiab tsuas yog siv sijhawm 1-2 vib nas this los so cov khoom txhua zaus. Txhawm rau txiav txim siab txog kev siv tshuaj tua kab mob ntawm cov khoom lag luam uas muaj tshuaj tua kab mob hauv hom kev so no, peb tau ua ib qho kev tshawb fawb ua ntej los ntsuas lub peev xwm ntawm ntau yam ntaub so uas muag los txo tus naj npawb ntawm cov kab mob tom qab so cov yas saum npoo ib zaug, peb zaug thiab tsib zaug. .
Npaj {{0}}.5 Maxwell turbidity (1.5) nyob rau hauv sterile ib txwm saline × 10 ^ 8 CFU/mL) ntawm MRSA, VRE thiab Pseudomonas aeruginosa, thiab 2.0 McDonnell turbidity ntawm Candida albicans. Txhawm rau simulate qhov chaw tsis muaj paug, ntxiv 100 μ Txhua kab kab mob L yuav tsum sib npaug thiab smeared hauv cov tais diav uas tsis muaj kab mob (10 cm inch), thiab tom qab ntawd qhuav lawm. Ntxiv nrog rau kev tswj cov tais diav, so cov tais diav nrog cov khoom hauv qab no:
Daim ntawv phuam da dej nrog ib txwm saline ( maj mam nyem daim ntawv phuam kom txog thaum nws tsis drips, tab sis tseem ntub dej);
5 feem pua ethanol ntub so;
Cov ntaub so ntub dej uas muaj 14.30 feem pua isopropanol thiab 0.23 feem pua quaternary ammonium ntsev;
0.5 feem pua hydrogen peroxide ntub phuam
Cov ntaub ntub dej uas muaj {{0}}.5 feem pua chlorhexidine thiab 70 feem pua isopropanol. So tag nrho cov tais diav nrog tib daim ntaub ntub dej rau 1, 3 lossis 5 zaug (txhua daim phuam ntub dej ua haujlwm li 1 thib ob), thiab cia nws qhuav rau 10 feeb. Tom qab ntawd ntxuav lub ntsej muag ntawm kab lis kev cai tais diav nrog 1mL trypsin soybean broth kom resuspension cov kab mob seem. Noj 100 μ L cov kab mob raug tshem tawm yog inoculated ntawm cov ntshav agar phaj los ntawm kev sau ntawv thiab kab lis kev cai ntawm 35 degree rau tsawg kawg 24 teev, thiab tom qab ntawd kev suav cov colony tau ua. Tag nrho cov txheej txheem tau ua tiav los ntawm tus kws tshawb fawb. Cov txiaj ntsig tau raug tshuaj xyuas los ntawm ib txoj kev ANOVA nrog Stata 9.0 (StataCorp, University Town, Texas).
Hauv txhua qhov kev sim, MRSA thiab VRE pawg tswj hwm muaj ntau ntau cov cheeb tsam suav rau ntawm lub phaj, yog li qhov feem pua ntawm cov cheeb tsam txo qis piv nrog xoom so tsis suav. Tom qab so qhov chaw rau peb lossis tsib zaug, tsib hom ntaub so tuaj yeem tua cov kab mob ntau dua li so ib zaug xwb (P<0.05; n="10)" (fig.="" 1).="" wiping="" the="" plastic="" surface="" with="" paper="" towels="" soaked="" in="" normal="" saline="" for="" three="" times="" can="" reduce="" more="" bacterial="" colonies="" than="" wiping="" only="" once,="" although="" this="" difference="" is="" not="" statistically="">
Cov txiaj ntsig tau pom tias tom qab so MRSA thiab VRE cov tais diav rau peb lossis tsib zaug, kev siv tshuaj tua kab mob ntawm tsib qhov kev sim cov ntaub so tsis muaj qhov sib txawv loj. Tom qab so ib zaug, tus nqi ntawm MRSA ntawm chlorhexidine cawv ntub cov ntaub so tau txo qis dua piv nrog cov ntaub so ntswg so nrog ib txwm saline; Piv nrog rau cov ntaub so ntswg so nrog cov ntsev ib txwm, cov quaternary ammonium ntsev compound so, chlorhexidine cawv so thiab hydrogen peroxide wipes tuaj yeem txo tus nqi ntawm VRE. (P<0.05; n="">
Vim tias muaj pes tsawg tus lej suav ntawm Candida albicans thiab Pseudomonas aeruginosa, lawv tsis raug txheeb xyuas. Txoj kev tshawb nrhiav pom tias txawm siv cov ntaub so zoo li cas (xws li cov ntaub so ntswg so hauv ib txwm saline), tus naj npawb ntawm cov kab mob ntawm cov yas yas tau txo qis nrog qhov nce ntawm cov sij hawm so. Piv nrog rau kev so ib zaug, so peb zaug tuaj yeem txo qhov nruab nrab ntawm cov kab mob los ntawm 88 feem pua. Thaum qhov chaw so rau 3 zaug lossis ntau dua, cov tshuaj tua kab mob ntawm cov ntaub so ntswg so hauv cov dej qab ntsev thiab cov tshuaj tua kab mob yog qhov zoo ib yam, uas zoo ib yam nrog cov txiaj ntsig kev tshawb fawb ntawm Rutala li al.: So lub computer keyboard yas tuav nrog dej muaj cov ntaub so rau 5 vib nas this tuaj yeem txo cov kab mob ua paug. Nyob rau hauv txoj kev tshawb no, tsuas yog thaum lub nto yog wiped ib zaug, lub sterilization nyhuv ntawm cov tshuaj tua kab mob yog zoo dua li ntawm cov ntaub so ntswg so nrog ib txwm saline.
Txawm hais tias txoj kev tshawb no tau ua tiav hauv chav kuaj, nws ntseeg tau tias cov txiaj ntsig kev sim tuaj yeem raug ntxiv rau qhov chaw kho mob ib puag ncig, vim tias cov kab mob inoculated hauv qhov kev sim no ntau dua li cov kab mob ib txwm muaj nyob rau hauv tsev kho mob cov khoom yas. Txawm li cas los xij, txhawm rau txhawm rau tshawb fawb zoo dua ntawm kev siv tshuaj ntxuav thiab tshuaj tua kab mob hauv thaj chaw kho mob, kev tshawb fawb yav tom ntej yuav tsum tau ua hauv thaj chaw kho mob los soj ntsuam thiab sib piv cov kev ntxuav, tshuaj tua kab mob thiab so cov khoom yas hauv tsev kho mob.
Hauv kev xaus, cov txiaj ntsig kev sim qhia tau hais tias txawm siv cov ntaub so zoo li cas los so cov yas, cov kab mob tuaj yeem txo tau. Thaum cov yas saum npoo yog so rau 3 zaug lossis ntau dua, cov tshuaj tua kab mob ntawm daim ntawv phuam so nrog cov ntsev ib txwm yog qhov zoo ib yam li cov ntaub so ntswg ntub dej. Txawm li cas los xij, yog tias cov neeg ua haujlwm kho mob tsuas yog so lub ntsej muag yas ib zaug xwb, lawv yuav tsum siv cov tshuaj tua kab mob.
